Dihydroethidium staining protocol
WebMar 15, 2024 · H&E. H&E is the most commonly used of all the various staining methods available in frozen section. H&E is simple to perform, inexpensive and reliable. The two … WebJan 16, 2013 · Superoxide anion (O 2 –) was detected in zebrafish freshly cut heart sections by DHE (Dihydroethidium) staining. DHE (Invitrogen, Carlsbad, CA), an oxidative fluorescent probe, was used to detect reactive oxygen species (ROS) in treated heart sections 2 h, 6 h and 14 h after H/R. Normoxic fishes were used as controls.
Dihydroethidium staining protocol
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WebAssay Protocol Short protocol: 1 -Adherent cells- Seed adherent cells at 25 x 103 per well one day before performing the assay. -Suspension cells- Grow suspension cells in … WebAdd DHE at final 10 μM (see protocol for DHE stock). (DHE IS VERY SENSITIVE TO LIGHT!!!) 11. Add the agonist for short term stimulation (Ang II, PDGF, etc.) 12. Incubate …
WebSep 2, 2024 · Dihydroethidium Staining. To assess the oxidative stress levels in the samples, the sections of the frozen kidney tissues from mice were stained with dihydroethidium (S0063, Beyotime, China) for 30 min. ... (R323-01, Vazyme, China), following the manufacturer’s protocol. Quantitative real-time PCR assays were … WebDihydroethidium. suitable for fluorescence, BioReagent, ≥95% (HPCE) View Price and Availability. Sigma-Aldrich. 309800. Dihydroethidium - CAS 38483-26-0 - Calbiochem. …
WebCentrifuge cells at 4°C and aspirate supernatant. Resuspend in 1ml of ice cold Hoechst staining buffer and incubate at 4°C for 15 minutes in the dark. Cell density should be between 4 x 10 5 and 1 x 10 6 cells per ml. Add 1.5µg of ethidium bromide for 15 minutes at 4°C. Run on flow cytometer. Sample histogram using this protocol. WebDihydroethidium staining was used to evaluate the reactive oxygen species (ROS).ResultsThe size of the AngII-induced AAA did not differ between pemafibrate- and vehicle-treated groups.
WebJul 5, 2024 · 5.5 ROS Staining Buffer 1. Dilute Dihydroethidium 1:1000 in Cell-Based Assay Buffer to prepare a working concentration of 5 µM (15 µL Dihydroethidium into …
WebApr 10, 2024 · PDF On Apr 10, 2024, Shubha Ranjan Dutta and others published hyp_hype-2024-20836_supp2.pdf Find, read and cite all the research you need on ResearchGate jewelry stores in breaux bridge laWebProtocol outline: Prepare standard curve samples and load into wells. Dilute experimental samples into reaction buffer and load into wells. Prepare the Amplex® Red reagent working solution. Add Amplex® Red solution to wells. Incubate. Measure fluorescence. Prepare reagent and add to cells. Incubate 20–60 min at 4–37°C depending on cell type. insta heat seal machineWebDihydroethidium Catalog Number D7008 Storage Temperature –20 °C CAS RN 104821-25-2 Molecular Formula: C 21H 21N 3 Molecular W eight: 315.4 Synonyms: 2,7-Diamino … jewelry stores in broadway mallWebDihydroethidium is designed for highly selective detection of superoxide in the mitochondria of live cells. ... 4.4.1 Protocol IV ... staining for F-actin and α-tubulin is highly recommended. For F-actin staining, fluorescently conjugated phalloidin is applied. A great variety of fluorophore–phalloidin conjugates is commercially available. jewelry stores in breckenridge coloradoWebProduct Description. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (λ Ex /λ Em: 355/420 nm) in cells, while … insta heat press reviewsWebProcedure Request a detailed protocol. Equal numbers of cells were plated in 8‐well plates. After treatment, cells were rinsed with PBS and incubated with 0.5 mmol/L dihydroethidium in PBS for 10 minutes at room temperature in the dark. Similarly, left ventricular sections were prepared using a cryostat on microscope slides. instaheat stromverbrauch testWebOct 15, 2015 · Therefore, I would like to know if anyone had experience using DHE with a fixation step and how it affects the staining. Also, which other methods could replace PFA fixation and guarantee bacteria ... jewelry stores in broadway square mall